DealsJust Now The interferon-alpha(IFN-alpha)-regulated hamster ISG-54 K gene, which is activated in hamster CHO-12 cells at least 40-fold, was isolated and the promoter region was characterized in detail. Sequence analysis revealed the presence of two elements, closely related to the interferon-stimulated-respon …
Deals5 hours ago Description. THP1-Dual™ cells feature the Lucia gene, a secreted luciferase reporter gene, under the control of an ISG54 minimal promoter in conjunction with five IFN-stimulated response elements. THP1-Dual™ cells also express a secreted embryonic alkaline phosphatase (SEAP) reporter gene driven by an IFN-β minimal promoter fused to five
Deals1 hours ago RAW-Lucia cells stably expressed an interferon sensitive response element from the mouse Isg54 minimal promoter and five interferon-stimulated response elements (Isre-Isg54…
Deals7 hours ago Interestingly, the ISG54 promoter does not have GAS elements but rather ISRE sequences that are bound by IRF3 dimers, IRF7 dimers, or ISGF3 transcriptions factors . However, our data clearly show that IFN-γ synergizes with transcription factors activated by poly I:C to induce the maximal expression of ISG54 in B16 melanoma cells.
Deals5 hours ago J774-Dual™ cells also express the Lucia luciferase gene, which encodes a secreted luciferase, under the control of an ISG54 minimal promoter in conjunction with five IFN-stimulated response elements. As a result, J774-Dual™ cells allow to simultaneously study the NF-κB pathway,
Deals3 hours ago IFN-β and ISG54 Promoter Reporter Gene Assays. HEK293T cells were transfected by using Lipofectamine 2000 (Invitrogen) with the indicated expression plasmids, an ISG54-promoter or an IFN-β-promoter firefly luciferase reporter plasmid (100 ng), and a constitutively expressed Renilla luciferase reporter plasmid, pRLTK (10 ng). At 18 hr
Deals1 hours ago This composite promoter is comprised of five IFN-stimulated response elements (ISRE) fused to an ISG54 minimal promoter, which is unresponsive to activators of …
Deals2 hours ago The Lucia gene, which codes for the secretion of coelenterazine-using luciferase, is driven by the ISG54 minimal promoter fused with six copies of NFAT shared transcription response elements. NFAT-Luc Reporter T lymphocytes respond to PMA and ionomycin and T lymphocyte mitogens, such as Concanavalin A (ConA) and phytohemagglutinin, to induce
Deals3 hours ago Inhibition of promoter activity by IRF3(5D) required the region located between nucleotides −6021 and −3636 (Fig. 5C). This region encompasses the conserved noncoding sequence (CNS)-2 enhancer . We then generated reporter constructs with a minimal proximal promoter (−301/+37) linked to different portions of this critical region.
Deals9 hours ago Mitochondrial antiviral signaling protein (MAVS) is strongly implicated as critical to control the replication of Ebola virus, but the role of MAVS in vivo is not known. Dutta et al. show that MAVS signaling specifically in myeloid cells controls replication of Ebola virus through both IFN-I-dependent and -independent mechanisms.
Deals7 hours ago THP1-Blue™ ISG cells express a secreted embryonic alkaline phosphatase (SEAP) reporter gene under the control of an ISG54 minimal promoter in conjunction with five IFN stimulated response elements. As a result, THP1-Blue™ ISG cells allow the monitoring of IRF activation by determining the activity of SEAP.
Deals1 hours ago Minimal Promoters for Custom Vectors VectorBuilder. (5 days ago) Sep 01, 2021 · minimal promoter s VectorBuilder offers many popular vector components that users can choose from when designing their vectors. The tables below provide detailed …. Via En.vectorbuilder.com. Category: Coupon , …
Deals7 hours ago THP1-Blue ISG cells (InvivoGen, San Diego, CA, USA), stably expressing a SEAP reporter gene under the control of an ISG54 minimal promoter with five IFN-stimulated response elements, were used to monitor interferon production by determining the activity of SEAP.
Deals5 hours ago A549-Dual™ cells also express the Lucia luciferase gene, which encodes a secreted luciferase, under the control of an ISG54 minimal promoter in conjunction with five IFN-stimulated response elements. As a result, A549-Dual™ cells allow to simultaneously study the NF-κB pathway,
Deals5 hours ago Minimal Heat Shock Promoter. Availability. Academic Institutions and Nonprofits only CENPW H3.3 gRNA. Plasmid #90620. Purpose. 3rd generation lentiviral gRNA plasmid targeting human CENPW. Depositor. Iain Cheeseman. Article. McKinley et al Dev Cell. 2017 Feb 27;40(4):405-420.e2.
Deals7 hours ago 74 an ISG54 minimal promoter in conjunction with five IFN-stimulated response elements. 75 Detectable levels of secreted luciferase as a read out for IFN activation was observed in a 76 temporal manner in response to Mtb Erdman at a MOI of 5 with 10-15 folds activity by 24h and 77 48h (Fig. 1A).
Deals7 hours ago By RT-qPCR analysis, we showed that ZIKV infection resulted in a minimal (ISG54/IFIT2 gene) to moderate (ISG56/IFIT1 gene) expression level of IFIT family genes in A549 Dual cells (Fig. 6A). There were no differences in ISG54/IFIT2 transcript levels with the three viral clones whereas MR766 MC and CHIM induced a stronger ISG56/IFIT1 gene
Deals3 hours ago The promoters for the four first exons of IRF5 contain different potential transcription factor binding sites. The 1A promoter contains putative binding sites for paired box 5 (PAX5), PU.1, SP1, and TCF12 which binds to enhancer boxes (E boxes). An extra SP1 binding site appears in those with the CGGGG 4× indel.
DealsJust Now (A) ISG56 and ISG54 inhibit SeV-induced activation of the IFN-β promoter in a dose-dependent manner. 293 cells (1 × 10 5) were transfected with an IFN-β promoter reporter (0.1 μg) and increased amount of ISG56 or ISG54 expression plasmids. Eighteen hours after transfection, cells were left uninfected or infected with SeV for 12 h before
Deals3 hours ago 1 or Stat2 to the endogenous ISG54 promoter. However, IFN -stimulated binding of RNA polymerase II to the ISG54 promoter is prevented by TSA. Interestingly, ec-topic expression of IRF9 reverses the inhibitory actions of TSA, suggesting that IRF9 functions to recruit RNA polymerase II to the promoter of interferon-stimulated genes.
Deals9 hours ago NFAT-Luc Reporter T Lymphocytes. Jurkat-Lucia ™ NFAT cells were derived from the human T Lymphocyte-based Jurkat cell line by stable integration of an NFAT-inducible Lucia reporter construct. The Lucia gene, which encodes a secreted coelenterazine-utilizing luciferase, is driven by an ISG54 minimal promoter fused to six copies of the NFAT consensus transcriptional response element.
Deals4 hours ago with MITA, we determined whether ISG56 and ISG54 regulate virus-triggered signaling. In reporter assays, overexpression of ISG56 strongly inhibited SeV-induced activation of the IFN-promoter in a dose-dependent manner in 293 cells, whereas overexpression of ISG54 …
Deals8 hours ago IFNβ treatment of fibroblasts induced the binding of both Stat1 and Stat2 to the ISG54 promoter (Fig. 3A, lanes 1, 2, 4, and 5) and of Stat1 to the IRF-1 promoter (Fig. 3B, lanes 1 and 2). TSA treatment of cells had no effect on the ability of either Stat to bind to the ISG54 (Fig. 3B, lanes 2, 3, 5, and 6) or IRF-1 (lanes 2 and 3) genes.
DealsJust Now cells were stably transduced with an IRF-inducible SEAP reporter construct under the control of the ISG54 minimal promoter in conjunction with five interferon-stimulated response elements (ISREs). D) ISG-inducing activity measured with THP1-Blue ISG. THP1-Blue …
Deals2 hours ago Human NF-kB-Luc Reporter T Lymphocytes also express a secreted embryonic alkaline phosphatase (SEAP) reporter gene, which works together with five IFN-stimulated response elements under the control of the ISG54 minimal promoter. As a result, JHuman NF-kB-Luc Reporter T Lymphocytes can simultaneously study the NF-κB pathway by monitoring the
Deals7 hours ago THP1-Lucia ISG cells expressed the secreted luciferase (Lucia) reporter gene under the control of an IRF-inducible promoter comprising five IFN-stimulated response elements (ISRE) fused to an ISG54 minimal promoter.
Deals8 hours ago HEK-Blue™ ISG cells express a secreted embryonic alkaline phosphatase (SEAP) under the control of the IRF-inducible promoter comprised of five IFN-stimulated response elements (ISRE) fused to an ISG54 minimal promoter.
Deals3 hours ago Introduction. Ionizing radiation (IR) induces cell damage via production of reactive oxygen species (ROS) and induction of DNA breaks. The altered microenvironment leads to changes in the expression of inflammatory cytokines, chemokines, and fibrotic cytokines, in cell–cell interactions, influx of inflammatory cells, and in induction of the reparative and restorative processes .
Deals3 hours ago For the first time we showed that the two closely related genes are expressed in a tissue-specific and inducer-specific manner. Furthermore, our findings provide the first evidence of a differential pattern of expression of ISG54 and ISG56 genes by IFN-α and IFN-β. The interferon (IFN) system is the first line of defense against virus
Deals2 hours ago Eventually, the effector cells release cytotoxic particles that kill the target cells. Jurkat NFAT-CD16 cells have been designed as effector reporter cells for our ADCC analysis. These cells stably express the Lucia luciferase reporter gene under the control of ISG54 minimal promoter fused with six NFAT response elements.
Deals8 hours ago These dual-reporter cells express the secreted embryonic alkaline phosphatase reporter gene under the control of the IFN-β minimal promoter fused to five NF-κB binding sites, as wells as the Lucia luciferase gene, which encodes a secreted luciferase, under the control of an ISG54 minimal promoter in conjunction with five IFN-stimulated
Deals3 hours ago the control of an IFNAR signaling-activated ISG54 minimal promoter, and THP1-Dual KO-IFNAR2 cells (blue columns), generated from THP1-Dual …
Deals1 hours ago They also express the secreted Lucia luciferase reporter gene under the control of an ISG54 minimal promoter in conjunction with five IFN-stimulated response elements. As a result, they allow to simultaneously study the NF-kB pathway, by assessing the activity of SEAP, and the interferon regulatory factor (IRF) pathway, by monitoring the
Deals9 hours ago promoter, whereas knockdown of ISG54 had a lesser effect (Fig. 3 H). These data suggest that endogenous ISG56 negatively. ments, the effects of ISG54 are minimal (Fig. 4 A and B). These
Deals9 hours ago Author summary Ionizing radiation is a powerful stressogenic factor that induces massive cell damage. The signals released from radiation-damaged tissues recruit the monocytes, which are differentiated into macrophages that remove dying cells via phagocytosis and facilitate inflammation but can also contribute to tumorigenesis through anti-inflammatory and regenerative activities.
Deals6 hours ago of ﬁve IFN-stimulated response elements (ISREs) fused to an ISG54 minimal promoter. (A and B) Cells were transduced with a lentiviral vector with a U3 deletion and expressing HA epitope-tagged
Deals8 hours ago Abstracts: AACR Special Conference on Tumor Immunology and Immunotherapy; November 17-20, 2019; Boston, MA Background: Agents that activate innate and adaptive immunity have the potential for transformative immunotherapeutic approach in cancer treatment. Recent evidence suggests that the induction of innate immunity via the activation of RIG-I pathway in tumor cells, as well as immune cells …
Deals2 hours ago We utilized THP-1 monocytes with an IFN reporter system to address this question. These cells secrete luciferase transcribed from a gene under the control of an ISG54 minimal promoter in conjunction with five ISREs. Using size exclusion chromatography, we isolated EVs from late passage CM that was combined from all 3 donors.
DealsJust Now Genes & Development
Deals4 hours ago A549-Dual™ cells were derived from A549 cells by stable integration of two reporter genes: SEAP gene (Secreted Embryonic Alkaline Phosphatase) and Lucia luciferase gene under the respective transcriptional control of an IFN-β minimal promoter, which is fused to NF-κB binding sites or an ISG54 minimal promoter in conjunction with interferon
Deals9 hours ago The human A549 lung carcinoma cell line expresses a secreted embryonic alkaline phosphatase (SEAP) reporter under the control of the IFN-β minimal promoter fused to five NF-κB binding sites, and a secreted luciferase under the control of an ISG54 minimal promoter in conjunction with five IFN-stimulated response elements (Invivogen).
Deals4 hours ago Hs00171042_m1; ISG54, Hs00533665_m1; ACTB, Hs99999903_m1). CT-DNA and poly(I·C) were from Sigma, LPS was from Santa Cruz Biotechnology, and antibodies were as follows: pY701-STAT1 and STAT1 (Cell Signaling Technology), anti-glyceraldehyde-3-phosphatedehydrogenase(VWR),andanti-p24(Abcam). Reporter assays.
Deals4 hours ago Indeed, transcriptional activity of STING-β promoter as reflected in the activation of luciferase reporter expression was detected in THP-1 cells and it was weaker than that of STING-α or SV40 promoter (Supplementary Figure S1A). The binding of STAT3 and IRF1 prompted us to …
Although ISG56 and ISG54 are among the first identified ISGs, little is known about their functions. It has been reported that ISG56 and ISG54 interact with the translation initiation factor eIF-3, leading to the inhibition of translation initiation and protein synthesis ( 22 ).
HEK-Blue™ ISG cells express a secreted embryonic alkaline phosphatase (SEAP) under the control of the IRF-inducible promoter comprised of five IFN-stimulated response elements (ISRE) fused to an ISG54 minimal promoter.
Overexpression of ISG56 inhibited Sendai virus-triggered activation of IRF3, NF-κB, and the IFN-β promoter, whereas knockdown of ISG56 had opposite effects. Consistently, overexpression of ISG56 reversed cytoplasmic poly (I:C)-induced inhibition of vesicular stomatitis virus (VSV) replication, whereas knockdown of ISG56 inhibited VSV replication.
THP1-Blue™ ISG cells were specifically designed to monitor the interferon (IFN) signaling pathway in a physiologically relevant cell line. They derive from the human THP-1 monocyte cell line by stable integration of an IFN regulatory factor (IRF)-inducible SEAP reporter construct.