Ef1a Promoter Sequence

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Addgene: IY704: pMVP (L1 R5) EF1a promoter Sequences

(1 days ago) Addgene: IY704: pMVP (L1-R5) EF1a promoter Sequences. GenBank File: Plasmid sequence and annotations. Use text editor or plasmid mapping software to view sequence. SnapGene File: Plasmid sequence and SnapGene enhanced annotations. Use with SnapGene software or the free Viewer to visualize additional data and align other sequences.

https://www.addgene.org/121693/sequences/

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The EF 1α promoter maintains high level transgene

(1 days ago) According to these findings, we concluded that the EF-1α promoter is a potent regulatory sequence for episomal vectors because it maintains high transgene expression, transgene stability and copy number. These results provide valuable information on improvement of transgene stability and the copy number of episomal vectors.

https://pubmed.ncbi.nlm.nih.gov/28557288/

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Addgene: pEF GFP

(Just Now) Plasmid pEF-GFP from Dr. Connie Cepko's lab contains the insert EF1 alpha promoter and is published in Proc Natl Acad Sci U S A. 2004 Jan 6. 101(1):16-22. This plasmid is available through Addgene.

https://www.addgene.org/11154/

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PLenti EF1a C mGFP Sequence and Map

(3 days ago) G A A T T C C T T A A G. BamHI (3831) 1 site. G G A T C C C C T A G G. After cleavage, BamHI-HF® (but not the original BamHI) can remain bound to DNA and alter its electrophoretic mobility. EF-1α promoter. 2624 .. 3802 = 1179 bp. strong constitutive promoter for human elongation factor EF-1α.

https://www.snapgene.com/resources/plasmid-files/?set=viral_expression_and_packaging_vectors&plasmid=pLenti-EF1a-C-mGFP

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Promoters of the day – EF1a diyspartanbiotech

(7 days ago) EF1a – a „strong“ native mammalian promoter active in most or all tissues. Not as strong as viral promoters like CMV. Tends not to get silenced (like CMV does). Sometimes the EF1a core promoter with CMV enhancer is used. Citing Invivogen “The EF-1 alpha gene encodes for elongation factor-1 alpha which is one of the…

https://diyspartanbiotech.wordpress.com/2017/02/04/promoters-of-the-day-ef1a/

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Lentiviral vector systems with an EF 1 alpha promoter

(3 days ago) Lenti-X lentiviral vector systems with EF-1 alpha promoters allow you to achieve robust, constitutive, long-term expression of your gene of interest in cell types in which CMV promoters are often silenced, such as hematopoietic and stem cells. The EF-1 alpha promoter, which offers a broad host range, is derived from the human EEF1A1 gene that expresses the alpha subunit of eukaryotic

https://www.takarabio.com/products/gene-function/viral-transduction/lentivirus/vector-systems/ef-1-alpha-promoter

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All Human EF1α Promoters Are Not Equal: Markedly Affect

(4 days ago) The promoter is a major element in the expression cassette of gene therapy vectors. Optimal promoter selection can enhance target specificity and gene expression. Recently, we evaluated three different human elongation factor 1 alpha (EF1 α) promoters. The three promoters were put

http://www.medsci.org/v11p0404.pdf

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Systematic Comparison of Constitutive Promoters and the

(7 days ago) promoter (EF1A), mouse phosphoglycerate kinase 1 promoter (PGK), and chicken b-Actin promoter coupled with CMV early Sequences for these promoters are given in Supporting Informa-tion, Text S1. Cells are obtained from the following sources: C2C12, MRC5, MEF, HT1080 and CMMT from ATCC

https://journals.plos.org/plosone/article/file?id=10.1371/journal.pone.0010611&type=printable

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Systematic Comparison of Constitutive Promoters and the

(3 days ago) Constitutive promoters are used routinely to drive ectopic gene expression. Here, we carried out a systematic comparison of eight commonly used constitutive promoters (SV40, CMV, UBC, EF1A, PGK and CAGG for mammalian systems, and COPIA and ACT5C for Drosophila systems). We also included in the comparison the TRE promoter, which can be activated by the rtTA transcriptional …

https://journals.plos.org/plosone/article?id=10.1371/journal.pone.0010611

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Systematic Comparison of the EF 1 Alpha Short (EFS) and

(9 days ago) MSCV promoter-driven vectors resulted in 33.76%, 33.1%, and 29% higher transgene expression at 5 ng, 10 ng, and 25 ng p24 equivalents compared with T cells transduced with vectors driven by the EFS promoter using the same amount of p24. After correction for integrated vector copy numbers, T cells had more than 2-fold increase in transgene

https://ashpublications.org/blood/article/124/21/3497/97941/Systematic-Comparison-of-the-EF-1-Alpha-Short-EFS

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EF1 promoter: pDRIVE vector with EF1 promoter (Ubiquitous

(6 days ago) These sequences have not been described yet. They share respectively 98.46, 47.73 and 45.05% homology with the sequence of the human EF-1α promoter. References: 1. Kim DW. et al. 1990. Use of human elongation factor 1 alpha promoter as a versatile and efficient expression system. Gene. 91(2):217-23. 2. Guo ZS. et al. 1996.

https://www.invivogen.com/pdrive-ef1

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The human elongation factor 1 alpha (EF 1 alpha) first

(1 days ago) The MCMV IE promoter was several fold stronger than t … The human elongation factor 1 alpha (EF-1 alpha) first intron highly enhances expression of foreign genes from the murine cytomegalovirus promoter J Biotechnol. 2002 Feb 14;93(2):183-7. doi: 10.1016/s0168-1656(01)00388-1.

https://pubmed.ncbi.nlm.nih.gov/11738725/

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Part:BBa K2520023 parts.igem.org

(9 days ago) EF1a promoter. Human elongation factor-1 alpha (EF-1 alpha) is a constitutive promoter of human origin. It can be used in-vitro and in-vivo to induce ectopic expression of recombinant genes. This promoter is very useful in cells where other promoters, such as CMV, are underactive or …

http://parts.igem.org/Part:BBa_K2520023

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Quantitative Comparison of Constitutive Promoters in Human

(3 days ago) The ACTB promoter was the most superior promoter in undifferentiated hESCs by maintaining transgene expression in 75–85% of the cells after 50 days in culture in both cell lines tested. In addition, the PGK promoter was found to express eGFP at high intensity up to 50 days of culture, whereas the intensities from the other promoters decreased

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2928720/

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Why EF1alpha promoter is so special? – SignaGen Blog

(3 days ago) Human elongation factor-1 alpha (EF-1 alpha) is a constitutive promoter of human origin that can be used to drive ectopic gene expression in various in vitro and in vivo contexts. EF-1 alpha is often useful in conditions where other promoters (such as CMV) have diminished activity or have been silenced (as in embryonic stem cells).

https://signagen.com/blog/2015/10/16/why-ef1alpha-promoter-is-so-special/

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EF1A Elongation factor 1 alpha Zea mays (Maize) EF1A

(8 days ago) At the electronic-microscope level, they are composed of heterogeneous mixture of electro-dense particles with diameters ranging from 20-25 nm and are called coiled body. Structures similar to CBs have been identified in the amphibian oocyte nucleus (called sphere organelles) and in …

https://www.uniprot.org/uniprot/Q41803

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The EF‐1α promoter maintains high‐level transgene

(9 days ago) The promoter and enhancer sequences are listed in Figure S2. The EF-1α and CAG promoters (Fig. S1D) were generated by polymerase chain reaction (PCR). To achieve directional cloning, Ase I and Nhe I restriction sites were introduced at the 5′ ends of the primers. The PCR cycling conditions were as follows: four cycles of 95°C for 3 min., 60

https://onlinelibrary.wiley.com/doi/full/10.1111/jcmm.13216

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Diyspartanbiotech The sky is not the limit

(Just Now) Not as strong as viral promoters like CMV. Tends not to get silenced (like CMV does). Sometimes the EF1a core promoter with CMV enhancer is used. Citing Invivogen “The EF-1 alpha gene encodes for elongation factor-1 alpha which is one of the most abundant proteins in eukaryotic cells and is expressed in almost all kinds of mammalian cells.

https://diyspartanbiotech.wordpress.com/

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EEF1A1 eukaryotic translation elongation factor 1 alpha 1

(8 days ago) This gene encodes an isoform of the alpha subunit of the elongation factor-1 complex, which is responsible for the enzymatic delivery of aminoacyl tRNAs to the ribosome. This isoform (alpha 1) is expressed in brain, placenta, lung, liver, kidney, and pancreas, and the other isoform (alpha 2) is expressed in brain, heart and skeletal muscle.

https://www.ncbi.nlm.nih.gov/gene/1915

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Promoters parts.igem.org

(2 days ago) A promoter is a DNA sequence that can recruit transcriptional machinery and lead to transcription of the downstream DNA sequence. The specific sequence of the promoter determines the strength of the promoter (a strong promoter leads to a high rate of transcription initiation). In addition to sequences that "promote" transcription, a promoter

https://parts.igem.org/Promoters

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Promoter associated RNA is required for RNA directed

(5 days ago) EF1a promoter-targeted siRNA consistently eluted with a low-copy RNA transcript corresponding to the EF1a promoter [supporting information (SI) Fig. 6]. This EF1a promoter-associated RNA was detected by reverse transcription (RT), using either a polydTT or an EF1a promoter-specific primer (Fig. 1A). The detection of the promoter-associated RNA in

https://www.pnas.org/content/pnas/104/30/12422.full.pdf

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Eukaryotic translation elongation factor 1 alpha 1 Wikipedia

(Just Now) Elongation factor 1-alpha 1 (eEF1a1) is a protein that in humans is encoded by the EEF1A1 gene.. This gene encodes an isoform of the alpha subunit of the elongation factor-1 complex, which is responsible for the enzymatic delivery of aminoacyl tRNAs to the ribosome.This isoform (alpha 1) is expressed in brain, placenta, lung, liver, kidney, and pancreas, and the other isoform (alpha 2) is

https://en.wikipedia.org/wiki/Eukaryotic_translation_elongation_factor_1_alpha_1

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PCas Guide EF1a CD4 – GE100022 OriGene

(3 days ago) Expresses gRNA containing the inserted target sequence and the gRNA scaffold EF1a promoter driven CD4 to enrich transfected cells CF3 primer ( GE100008 ) can be used to sequence the target sequence

https://www.origene.com/catalog/vectors/crispr-vectors/ge100022/pcas-guide-ef1a-cd4

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Ef1a Promoter Sequence mybestcouponcodes.com

(7 days ago) The EF-1α promoter maintains high-level transgene . CODES (1 days ago) According to these findings, we concluded that the EF-1α promoter is a potent regulatory sequence for episomal vectors because it maintains high transgene expression, transgene stability and copy number. These results provide valuable information on improvement of transgene stability and the copy number of episomal vectors.

https://www.mybestcouponcodes.com/ef1a-promoter-sequence/

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EEF1A1 Elongation factor 1 alpha 1 Homo sapiens (Human

(8 days ago) This protein promotes the GTP-dependent binding of aminoacyl-tRNA to the A-site of ribosomes during protein biosynthesis. Plays a role in the positive regulation of IFNG transcription in T-helper 1 cells as part of an IFNG promoter-binding complex with TXK and PARP1 (PubMed:17177976).

https://www.uniprot.org/uniprot/P68104

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Promoter associated RNA is required for RNA directed

(1 days ago) To ascertain the requirement of the promoter-associated RNA variants for the induction of siRNA-mediated TGS, we performed a blocking experiment in which antisense phosphorothioate oligodeoxynucleotides (ODNs) were designed to recognize the cognate promoter-associated RNA variant sequence for either the EF1a or CCR5 promoters.

https://www.pnas.org/content/104/30/12422

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Lentiviral vectors carrying enhancer elements of Hb9

(7 days ago) With EF1a promoter GFP expression is detected with similar intensity in neuronal cells (arrow in a) and in astrocytes (arrowheads in a, b), whereas, on the other hand, with rNSE promoter the expression is mainly restricted to neurons (arrows in c, d). Scale bar in (d) applies to (a–c) = 100 μm.

https://www.sciencedirect.com/science/article/pii/S0165027012000027

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PBApo EF1(alpha) Neo Sequence and Map

(5 days ago) pBApo-EF1 (alpha) Neo.dna. Map and Sequence File: Download Open. Sequence Author: TaKaRa. Download Free Trial Get SnapGene Viewer.

https://www.snapgene.com/resources/plasmid-files/?set=mammalian_expression_vectors&plasmid=pBApo-EF1(alpha)_Neo

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MiRNA Related Products and Services Provided by Biosettia

(8 days ago) The human EF1a promoter, used to express miRNA precursors and the puromycin selection marker, is a house-keeping gene promoter. Therefore, it is unlikely to be silenced by methylation in vitro and in vivo. Our rPuro gene product, expressed from the EF1a promoter, is the red fluorescent puromycin-N …

https://biosettia.com/mirna/

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Promoter (genetics) Wikipedia

(3 days ago) In genetics, a promoter is a sequence of DNA to which proteins bind that initiate transcription of a single RNA from the DNA downstream of it. This RNA may encode a protein, or can have a function in and of itself, such as tRNA, mRNA, or rRNA.Promoters are located near the transcription start sites of genes, upstream on the DNA (towards the 5' region of the sense strand).

https://en.wikipedia.org/wiki/Gene_promoter

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EEF1A1 Gene GeneCards EF1A1 Protein EF1A1 Antibody

(7 days ago) EEF1A1 (Eukaryotic Translation Elongation Factor 1 Alpha 1) is a Protein Coding gene. Diseases associated with EEF1A1 include Eukaryotic Translation Elongation Factor 1 Alpha-1-Like 14 and Felty Syndrome.Among its related pathways are Cellular Senescence (REACTOME) and Metabolism of proteins.Gene Ontology (GO) annotations related to this gene include protein kinase binding.

https://www.genecards.org/cgi-bin/carddisp.pl?gene=EEF1A1

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Ef1a PCR/Sanger Sequencing Primer pairs

(3 days ago) TaqMan™ Advanced miRNA Assays, based on a universal RT approach, provide even greater sensitivity in biological fluids and proven TaqMan™ specificity. TaqMan™ MicroRNA Assays, based on the specific stem-loop primer approach, are the gold standard for sensitivity and specificity. The miRNA control assays are unique to each assay chemistry

https://www.thermofisher.com/order/genome-database/browse/pcr-sequencing-primers/keyword/EF1a

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Vector Map Takara Bio

(2 days ago) • cPPT/CTS (central polypurine tract/central termination sequence): 2028–2151 • PEF1α (human elongation factor 1 alpha promoter): 2185–3519 • MCS (multiple cloning site): 3535–3572 • IRES (internal ribosome entry site): 3573–4148 • Puror (puromycin resistance gene): 4181–4780

https://www.takarabio.com/assets/documents/Vector%20Documents/pLVX-EF1alpha-IRES-Puro%20Vector%20Information.pdf

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PAV EF1a, PM10005 Vigene Biosciences

(4 days ago) Promoter: EF1a: Storage Buffer: TE Buffer: Storage Conditions-20°C: Shipping: Ambient temperature: Use Restrictions . For Research Use Only. Plasmid Protocol . PDF (557.6 KB) Plasmid Map . PNG (30.3 KB) Sequence . DNA (52.5 KB) Related Products . pAV-ALB. AAV vector with ALB promoter. Read More > pAV-GFAP104. AAV vector with GFAP104

https://www.vigenebio.com/pav-ef1a

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AAV with EF1a promoter driven EGFP T2A iCre Vector Biolabs

(9 days ago) Name: AAV-EF1a-EGFP-T2A-iCre. This AAV expresses EGFP-T2A-iCre driven by an ubiquitous EF1a promoter. The 1.2 Kb EF1α promoter is a commonly used, constitutive promoter derived from human elongation factor-1 alpha (EF1α). It is often used in conditions where the other constitutive promoters, such as CMV, have diminished activity or have been

https://www.vectorbiolabs.com/product/vb2069-aav-with-ef1a-promoter-driven-egfp-t2a-icre/

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Small Interfering RNA Induced Transcriptional Gene

(7 days ago) We chose to target an elongation factor 1 alpha (EF1A) promoter–green fluorescent protein (GFP) reporter gene integrated into the genome of human 293FT cells by transduction with a feline immunodeficiency virus (FIV) vector . siRNA EF52 is homologous to an EF1A promoter sequence essential for transcription (AAG GTG GCG CGG GGT AAA CTG, –106

https://science.sciencemag.org/content/305/5688/1289.full

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A bidirectional promoter architecture enhances lentiviral

(Just Now) Here, we demonstrate that a back-to-back configuration of the mouse Pgk1 and human eukaryotic translation elongation factor 1 alpha 1 promoters provided a substantive increase in …

https://www.nature.com/articles/gt201126

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E2F1 an overview ScienceDirect Topics

(4 days ago) The E2F1 promoters from mouse or human cells contain four overlapping E2F binding sites within 30 bp upstream of the transcription start site (Fig. 5) (59–61).Both promoters contain several Sp1 sites within 200 bp of the start site as well as an Sp1 site downstream of the start site, and one or more CCAAT sequences.The function of these elements was investigated in quiescent and serum

https://www.sciencedirect.com/topics/neuroscience/e2f1

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Vector Detail plasmid.med.harvard.edu

(1 days ago) Vector: pHAGE-shortEF1a-MCS-IRES-ZsGreen-W. Number of plasmids with this backbone: 1. Name: pHAGE-shortEF1a-MCS-IRES-ZsGreen-W. Description: Lentiviral vector that expresses insert from internal short EF1a promoter, after MCS for insert is IRES-ZsGreen fluorescent marker, with WPRE; ampicillin resistance; restriction enzyme cloning. Synonyms:

https://plasmid.med.harvard.edu/PLASMID/GetVectorDetail.do?vectorid=252

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PCas Guide EF1a GFP – GE100018 OriGene

(3 days ago) USD 125.00. Add To Cart. TP790151. Purified recombinant protein of mutant (D10A) of S. pyogenes Cas9 endonuclease (Cas9) containing Simian virus 40 (SV40) T antigen nuclear localization sequence (NLS) on the N- and C- termini of the protein., with N-terminal HIS tag, expressed in E. coli. 500 pmol.

https://www.origene.com/catalog/vectors/crispr-vectors/ge100018/pcas-guide-ef1a-gfp

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EF1a What does EF1a stand for? The Free Dictionary

(3 days ago) Looking for online definition of EF1a or what EF1a stands for? EF1a is listed in the World's largest and most authoritative dictionary database of abbreviations and acronyms The Free Dictionary

https://acronyms.thefreedictionary.com/EF1a

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FAQ?

Which is the core promoter of the EF1A gene??

Sometimes the EF1a core promoter with CMV enhancer is used. Citing Invivogen “The EF-1 alpha gene encodes for elongation factor-1 alpha which is one of the most abundant proteins in eukaryotic cells and is expressed in almost all kinds of mammalian cells.

Is the ef1α promoter the same as luciferase cDNA??

Recently, we evaluated three different human elongation factor 1 alpha (EF1α) promoters. The three promoters were put into the same expression vector, pAC-luc, driving expression of the luciferase cDNA.

Is the EF1 promoter ubiquitous in PDRIVE expression??

EF1 promoter (Ubiquitous) in pDRIVE expression plasmid The EF-1 alpha gene encoding elongation factor-1 alpha is an enzyme which catalyzes the GTP-dependent binding of aminoacyl-tRNA to ribosomes. EF-1α is one of the most abundant proteins in eukaryotic cells and is expressed in almost all kinds of mammalian cells.

Where can I find the EF1 alpha promoter??

This material is available to academics and nonprofits only. The EF1 alpha promoter (Kim et al., Gene 91, 217-273 (1990)) was obtained from Dr. S. Sugano (Univ. of Tokyo). These plasmids were created by your colleagues.

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